Quantum dots-hemin: Preparation and application in the absorption of heme iron

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• 作者：Lina Geng ; PhD^a ; Xianglin Duan ; BS^b ; ¹ ; Yan Wang ; MD^b ; Yashuo Zhao ; PhD^b ; Guofen Gao ; PhD^b ; Delong Liu ; PhD^a ; Yan-Zhong Chang ; PhD^b ; ^{chang7676@163.com" class="auth_mail" title="E-mail the corresponding author} ; Peng Yu ; PhD^b ; ^{yupeng0311@mail.hebtu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{yupeng0311@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Heme ; Quantum dots ; Heme carrier protein 1 ; Micro X-ray fluorescence ; Intestine
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：12
• 期：7
• 页码：1747-1755
• 全文大小：1451 K

文摘

The absorption mechanism of heme iron remains unclear due to the limit of labeling techniques. Quantum dots (QDs) are powerful fluorescent probes resistant to photobleaching, however, there is no data about the application of QDs in heme iron absorption. Herein, we prepared hemin-coated CdSe/ZnS (QDs-hemin), and studied their absorption in vitro and in vivo. Results showed that QDs-hemin had uniform particle sizes, physiological stability and high joint efficiency. Moreover, QDs-hemin could be successfully absorbed gradually into the duodenum with the time using synchrotron radiation micro X-ray fluorescence and confocal laser scanning microscopy. Furthermore, QDs-hemin were observed to degrade in lysosomes, and their absorption was blocked by Heme Carrier Protein 1 (HCP1) antibody and HCP1 siRNA. All the results demonstrate that QDs can be a good tracer for heme iron and that HCP1 pathway is critical and predominant over the endocytosis pathway in the absorption mechanism.