Production and characterization of a biotinylated single-chain variable fragment antibody for detection of parathion-methyl
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- 作者：Huimin Wang1 ; Fengchun Zhao1 ; Xiao Han ; Zhengyou Yang ; zhyouyang@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Parathion-methyl ; Phage display ; Biotinylation ; scFv antibody ; IC-ELISA
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：126
- 期：Complete
- 页码：1-8
- 全文大小：1455 K
文摘
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A pre-immunized phage display library was constructed against parathion-methyl.
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ScFv antibodies were expressed and selected by an indirect competitive ELISA.
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The selected antibody was expressed in high-level and biotinylated in vitro by BirA.
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The biotinylated antibody was confirmed by SDS-PAGE and western blotting.
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A sensitive and specific IC-ELISA for parathion-methyl was developed.