- 作者：Kaku Nakano ; PhD?/sup> ; Kensuke Egashira ; MD ; PhD?/sup> ; egashira@cardiol.med.kyushu-u.ac.jp ; Seigo Masuda ; MD?/sup> ; Kouta Funakoshi ; MD?/sup> ; Gang Zhao ; MD ; PhD§ ; ; Satoshi Kimura ; MD&dagger ; ; Tetsuya Matoba ; MD ; PhD?/sup> ; Katsuo Sueishi ; MD ; PhD&Dagger ; ; Yasuhisa Endo ; PhD¶ ; ; Yoshiaki Kawashima ; PhD?/sup> ; Kaori Hara ; PhD# ; Hiroyuki Tsujimoto ; PhD# ; Ryuji Tominaga ; MD ; PhD&dagger ; ; Kenji Sunagawa ; MD ; PhD?/sup>
- 关键词：drug delivery system ; nanotechnology ; restenosis ; smooth muscle cells ; stents
- 刊名：JACC: Cardiovascular Interventions
- 出版年：2009
- 出版时间：April 2009
- 年：2009
- 卷：2
- 期：4
- 页码：277-283
- 全文大小：1889 K
Objectives
The objective of this study was to formulate a nanoparticle (NP)-eluting drug delivery stent system by a cationic electrodeposition coating technology.Background
Nanoparticle-mediated drug delivery systems (DDS) are poised to transform the development of innovative therapeutic devices. Therefore, we hypothesized that a bioabsorbable polymeric NP-eluting stent provides an efficient DDS that shows better and more prolonged delivery compared with dip-coating stent.
Methods
We prepared cationic NP encapsulated with a fluorescence marker (FITC) by emulsion solvent diffusion method, succeeded to formulate an NP-eluting stent with a novel cation electrodeposition coating technology, and compared the in vitro and in vivo characteristics of the FITC-loaded NP-eluting stent with dip-coated FITC-eluting stent and bare metal stent.
Results
The NP was taken up stably and efficiently by cultured vascular smooth muscle cells in vitro. In a porcine coronary artery model in vivo, substantial FITC fluorescence was observed in neointimal and medial layers of the stented segments that had received the FITC-NP-eluting stent until 4 weeks. In contrast, no substantial FITC fluorescence was observed in the segments from the polymer-based FITC-eluting stent or from bare metal stent. The magnitudes of stent-induced injury, inflammation, endothelial recovery, and neointima formation were comparable between bare metal stent and NP-eluting stent groups.
Conclusions
Therefore, this NP-eluting stent is an efficient NP-mediated DDS that holds as an innovative platform for the delivery of less invasive nano-devices targeting cardiovascular disease.