In this study, the complete mitochondrial genome sequence of Triplophysa lixianensis was determined.
The sequences of all 22 tRNA genes of T. lixianensis can fold into the standard cloverleaf secondary structure.
The phylogenetic status of T. lixianensis was confirmed.
We proved that Triplophysa and Hedinichthys can not unit as a same genus used the mitochondrial genome sequences.