Transmissible gastroenteritis virus infection induces apoptosis through FasL- and mitochondria-mediated pathways

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• 作者：Li Ding¹ ; Xingang Xu¹ ; Yong Huang ; Zhaocai Li ; Kuan Zhang ; Guangda Chen ; Gaoshui Yu ; Zhisheng Wang ; Wei Li ; Dewen Tong ; ^{dwtong@nwsuaf.edu.cn}
• 关键词：TGEV ; Apoptosis ; FasL ; Mitochondria ; Viral replication
• 刊名：Veterinary Microbiology
• 出版年：2012
• 出版时间：6 July, 2012
• 年：2012
• 卷：158
• 期：1-2
• 页码：12-22
• 全文大小：1599 K

文摘

Transmissible gastroenteritis virus (TGEV) has been reported to induce apoptosis in swine testis (ST) cells. However, the mechanisms underlying TGEV-induced apoptosis are still unclear. In this study we observed that TGEV infection induced apoptosis in porcine kidney (PK-15) cells in a time- and dose-dependent manner. TGEV infection up-regulated FasL, activated FasL-mediated apoptotic pathway, leading to activation of caspase-8 and cleavage of Bid. In addition, TGEV infection down-regulated Bcl-2, up-regulated Bax expression, promoted translocation of Bax to mitochondria, activated mitochondria-mediated apoptotic pathway, which in turn caused the release of cytochrome c and the activation of caspase-9. Both extrinsic and intrinsic pathways activated downstream effector caspase-3, followed by the cleavage of PARP, resulting in cell apoptosis. Moreover, TGEV infection did not induce significant DNA fragmentation in ammonium chloride (NH₄Cl) pretreated PK-15 cells or cells infected with UV-inactivated TGEV. In turn, block of caspases activation also did not affect TGEV replication. Taken together, this study demonstrates that TGEV-induced apoptosis is dependent on viral replication in PK-15 cells and occurs through activation of FasL- and mitochondria-mediated apoptotic pathways.