Acetic acid-induced programmed cell death and release of volatile organic compounds in Chlamydomonas reinhardtii

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• 作者：Zhaojiang Zuo ; Yerong Zhu ; Yanling Bai ; Yong Wang ; ^{wangyong@nankai.edu.cn}
• 关键词：Acetic acid ; Cell death effect ; Chlamydomonas reinhardtii ; Programmed cell death ; Volatile organic compounds
• 刊名：Plant Physiology and Biochemistry
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：51
• 期：Complete
• 页码：175-184
• 全文大小：962 K

文摘

Acetic acid widely spreads in atmosphere, aquatic ecosystems containing residues and anoxic soil. It can inhibit aquatic plant germination and growth, and even cause programmed cell death (PCD) of yeast. In the present study, biochemical and physiological responses of the model unicellular green algae Chlamydomonas reinhardtii were examined after acetic acid stress. H₂O₂ burst was found in C.?reinhardtii after acetic acid stress at pH 5.0 for 10?min. The photosynthetic pigments were degraded, gross photosynthesis and respiration were disappeared gradually, and DNA fragmentation was also detected. Those results indicated that C.?reinhardtii cells underwent a PCD but not a necrotic, accidental cell death event. It was noticed that C.?reinhardtii cells in PCD released abundant volatile organic compounds (VOCs) upon acetic acid stress. Therefore, we analyzed the VOCs and tested their effects on other normal cells. The treatment of C.?reinhardtii cultures with VOCs reduced the cell density and increased antioxidant enzyme activity. Therefore, a function of VOCs as infochemicals involved in cell-to-cell communication at the conditions of applied stress is suggested.