pH-dependent conformational changes of ferricytochrome c induced by electrode surface microstructure

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• 作者：Jiang ; Xiue ; Qu ; Xiaohu ; Zhang ; Lei ; Zhang ; Zheling ; Jiang ; Junguang ; Wang ; Erkang ; Dong ; Shaojun
• 关键词：CD spectra ; SWNTs modified electrode ; Electrode surface microstructure ; Alkaline conformational changes of ferricytochrome c
• 刊名：Biophysical Chemistry
• 出版年：2004
• 出版时间：August 1, 2004
• 年：2004
• 卷：110
• 期：3
• 页码：203-211
• 全文大小：390 K

文摘

pH-dependent processes of bovine heart ferricytochrome c have been investigated by electronic absorption and circular dichroism (CD) spectra at functionalized single-wall carbon nanotubes (SWNTs) modified glass carbon electrode (SWNTs/GCE) using a long optical path thin layer cell. These methods enabled the pH-dependent conformational changes arising from the heme structure change to be monitored. The spectra obtained at functionalized SWNTs/GCE reflect electrode surface microstructure-dependent changes for pH-induced protein conformation, pK_a of alkaline transition and structural microenvironment of the ferricytochrome c heme. pH-dependent conformational distribution curves of ferricytochrome c obtained by analysis of in situ CD spectra using singular value decomposition least square (SVDLS) method show that the functionalized SWNTs can retain native conformational stability of ferricytochrome c during alkaline transition.