In situ circular dichroic electrochemical study of bilirubin and bovine serum albumin complex

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• 作者：Bai ; Hanying ; Liu ; Xiaoqiang ; Zhang ; Zheling ; Dong ; Shaojun
• 关键词：Bilirubin ; Bovine serum albumin ; Circular dichroism ; Enantiomer of bilirubin ; Conformation transition of bilirubin
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2004
• 出版时间：January, 2004
• 年：2004
• 卷：60
• 期：1-2
• 页码：155-160
• 全文大小：142 K

文摘

The electrooxidation of bilirubin (BR) and bovine serum albumin (BSA) complexes was studied by in situ circular dichroism (CD) spectroelectrochemistry. The result showed that the mechanism of the whole electrooxidation process of this complex corresponded to electrochemical processes (EE mechanism) in aqueous solution. Some parameters of the process were obtained by double logarithm method, differential method and nonlinear regression method. In visible region, CD spectra of the two enantiomeric components of the complex and their fraction distribution against applied potentials were obtained by singular value decomposition least-square (SVDLS) method. Meanwhile, the distribution of the five components of secondary structure was also obtained by the same method in far-UV region. The peak potential gotten from EE mechanism corresponds to a turning point for the component transition, beyond which the whole reaction reaches a new equilibrium. Under applied positive potentials, the enantiomeric equilibrium between M and P form is broken and M form transfers to its enantiomer of P, while the fraction of α-helix increases and that improves the transition to P form.