Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide in Bacillus subtilis
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- 作者：Chengran Guan1 ; Wenjing Cui1 ; 2 ; ; Jintao Cheng1 ; Rui Liu1 ; Zhongmei Liu1 ; 2 ; Li Zhou1 ; 2 ; Zhemin Zhou1 ; 2 ; zhmzhou@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 刊名：New Biotechnology
- 出版年：2016
- 出版时间：25 May 2016
- 年：2016
- 卷：33
- 期：3
- 页码：372-379
- 全文大小：311 K
文摘
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PgsiB–PHpaII was prominent among the single and dual promoters evaluated.
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SPYncM was selected from 19 signal peptides to optimize the secretion efficiency.
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The capacity of the expression system was demonstrated with batch fermentation.