Activity and substrate specificity of phospholipase A1 in hexane media were enhanced by bio-imprinting.
Activity of imprinted-phospholipase A1 in hexane media was further improved by immobilization on diatomite.
Crude soybean oil could be highly efficiently degummed by immobilized-imprinted-phospholipase A1.
Immobilized-imprinted-phospholipase A1 could be used in oil/hexane miscella degumming before desolventizing.