Antiproliferation and apoptosis of human tumor cell lines by a lectin (AMML) of Astragalus mongholicus

详细信息    查看全文

• 作者：Qiaojuan Yan ; Yanxia Li ; Zhengqiang Jiang ; Yan Sun ; Lifen Zhu ; Zufeng Ding
• 关键词：Antitumor ; Apoptosis ; Astragalus mongholicus ; Lectin ; Cell cycle arrest ; Cell proliferation
• 刊名：Phytomedicine
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：16
• 期：6-7
• 页码：586-593
• 全文大小：364 K

文摘

A lectin (AMML) from the roots of Astragalus mongholicus was extracted and purified by affinity chromatographic technique. Human cervical carcinoma cell line (HeLa), human osteoblast-like cell line (MG63) and human leukemia cell line (K562) were used to check the effects of AMML on cell proliferation, apoptosis and cell cycle. Maximum growth inhibition (92 % ) was observed with HeLa cells, followed by K562 cells (84 % ) and MG63 (48 % ) cells. Morphological observation showed that AMML-treated HeLa cells displayed outstanding apoptosis characteristics, such as nuclear fragmentation and appearance of membrane-enclosed apoptotic bodies. The apoptosis of HeLa cells was confirmed by flow cytometry using Annexin V/FITC and propidium iodide (PI) staining technique. For the first time we also report a significant cell cycle arrest at S phase of HeLa cells by AMML. Therefore, the present investigation may lead to the possible therapeutic use of Astragalus mongholicus lectin.