Gene cloning, functional expression and characterisation of a novel glycogen branching enzyme from Rhizomucor miehei and its application in wheat breadmaking
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- 作者：Shupeng Wua ; Yu Liub ; Qiaojuan Yanb ; Zhengqiang Jianga ; zhqjiang@cau.edu.cn" class="auth_mail
- 关键词：DP ; degree of polymerisation ; GH ; glycoside hydrolase ; HPAEC ; high performance anionic-exchange chromatography ; RACE ; Rapid Amplification of cDNA Ends ; MES ; 2-(N-morpholino) ethane sulphonic acid ; RmGBE ; 伪-1 ; 4-glycogen branching enzyme from Rhizomucor miehei
- 刊名：Food Chemistry
- 出版年：15 September 2014
- 年：2014
- 卷：159
- 期：Complete
- 页码：85-94
- 全文大小：3035 K
文摘
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A fungal glycogen branching enzyme from Rhizomucor miehei was cloned and expressed.
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The enzyme was characterised and displayed excellent cold adaptation.
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The enzyme exhibited ten times higher activity toward amylose than amylopectin.
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The enzyme was found to improve bread quality and increase its shelf life.