Quantification of peramivir in dog plasma by liquid chromatography/tandem mass spectrometry employing precolumn derivatization
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- 作者：Xin Li ; Ying Li ; Juan Wang ; Lili Wang ; Wu Zhong ; Jinxiu Ruan ; Zhenqing Zhang
- 关键词：Peramivir ; Influenza virus neuraminidase inhibitor ; Derivatization ; Liquid chromatography&ndash ; tandem mass spectrometric method (LC&ndash ; MS/MS) ; Dog plasma
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：1 January, 2014
- 年：2014
- 卷：944
- 期：Complete
- 页码：1-5
- 全文大小：556 K
文摘
Peramivir is a novel influenza neuraminidase inhibitor used for anti-influenza. In this article, a novel method was developed to determine peramivir in dog plasma using a derivatization treatment step to increase the retention time and enhance the signal intensity. The sample preparation consisted of a protein precipitation extraction followed by derivatization with 10 M hydrochloric acid-methanol (10:90, v/v) and determined by liquid chromatography coupled with tandem mass spectrometry. The selected reaction monitoring mode of the positive ion was performed and the precursor to the product ion transitions of m/z 343 鈫?#xA0;284 and m/z 299 鈫?#xA0;152 were used to measure the derivative of peramivir and Ro 64-0802 (internal standard, an active metabolite of oseltamivir). The chromatographic separation was achieved using a ZORBAX RX-C8 (2.0 mm 脳 150 mm 脳 5 渭m) analytical column with an isocratic mobile phase composed of acetonitrile-water-formic acid (30:70:0.1, v/v/v, 0.2 mL/min). The method was linear over a concentration range of 0.25-250 ng/mL. The average intra-day/inter-day precision values were 4.04-8.17% and 3.02-7.08%, respectively, while the average accuracy value was 93.99-106.48%. This method has been successfully applied to the preclinical dog research of peramivir following intragastric administration.