Fully human broadly neutralizing monoclonal antibodies against influenza A viruses generated from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient

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• 作者：Weibin Hu^a ; ¹ ; Aizhong Chen^b ; ¹ ; Yi Miao^c ; ¹ ; Shengli Xia^d ; ¹ ; Zhiyang Ling^a ; Ke Xu^a ; Tongyan Wang^a ; Ying Xu^b ; Jun Cui^b ; Hongqiang Wu^b ; Guiyu Hu^b ; Lin Tian^b ; Lingling Wang^b ; Yuelong Shu^e ; Xiaowei Ma^f ; Bianli Xu^d ; Jin Zhang^d ; Xiaojun Lin^f ; ^{linxiaojun@hualan.com} ; Chao Bian^b ; ^{cbian@sibs.ac.cn} ; Bing Sun^a ; ^b ; ^{bsun@sibs.ac.cn}
• 关键词：mAb ; monoclonal antibody ; TCID50 ; 50 % tissue culture infective dose
• 刊名：Virology
• 出版年：2013
• 出版时间：20 January, 2013
• 年：2013
• 卷：435
• 期：2
• 页码：320-328
• 全文大小：1266 K

文摘

Whether the 2009 pandemic H1N1 influenza vaccine can induce heterosubtypic cross-protective anti-hemagglutinin (HA) neutralizing antibodies is an important issue. We obtained a panel of fully human monoclonal antibodies from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient. Most of the monoclonal antibodies targeted the HA protein but not the HA1 fragment. Among the analyzed antibodies, seven mAbs exhibited neutralizing activity against several influenza A viruses of different subtypes. The conserved linear epitope targeted by the neutralizing mAbs (FIEGGWTGMVDGWYGYHH) is part of the fusion peptide on HA2. Our work suggests that a heterosubtypic neutralizing antibody response primarily targeting the HA stem region exists in recipients of the 2009 pandemic H1N1 influenza vaccine. The HA stem region contains various conserved neutralizing epitopes with the fusion peptide as an important one. This work may aid in the design of a universal influenza A virus vaccine.