Effects of EGF on apoptosis of adipose derived stem cells by regulating miRNA-21

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• 作者：Ying Liu^a ; ¹ ; Xi Chen^b ; ¹ ; Shuai Qiang^a ; ¹ ; Qiushi Lin^c ; Fuliang He^b ; ^c ; Xiaoqun Dong^c ; ^{Xiaoqun-Dong@ouhsc.edu" class="auth_mail" title="E-mail the corresponding author} ; Zhibo Xiao^a ; ^{xiaozhibodoctor@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Adipose tissue-derived stem cells (ADSCs) ; EGF ; MiR-21 ; PTEN ; FASL
• 刊名：Wound Medicine
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：12
• 期：Complete
• 页码：10-14
• 全文大小：1040 K

文摘

The miroRNA-21 (MiR-21) plays a vital role in the process of proliferation and apoptosis in different types of cells. EGF could increase the miR-21 primary transcript during adipose derived stem cells (ADSCs) differentiation, however, the underlying molecular mechanism is unclear. We hypothesized that EGF affects the proliferation and apoptosis of ADSCs by regulating miRNA-21.

Methods

Gain or loss of function strategy was used through transfecting ADSCs with chemically synthesized oligonucleotides complementary to miR-21. Profiles of post-transfection miR-21 caused by EGF were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Apoptosis of ADSCs was evaluated with flow cytometry. The expression levels of miR-21's targeted genes PTEN and FASLwere determined by Western blot assay.

Results

In response to miR-21 mimics or inhibitor, the apoptosis and cytoactivity of ADSCs changed correspondingly. Change of miR-21 expression level was checked and an inverse correlation of EGF was observed with PTEN and FASL expression in ADSCs.

Conclusion

Our results indicated that miR-21 could influence EGF expression and furtherly inhibits the apoptosis of ADSCs by downregulating PTEN and FASL. These findings may provide clues toward regulating biological activity of ADSCs with miR-21.