Structural and functional properties of alkali-treated high-amylose rice starch
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- 作者：Jinwen Cai ; Yang Yang ; Jianmin Man ; Jun Huang ; Zhifeng Wang ; Changquan Zhang ; Minghong Gu ; Qiaoquan Liu ; Cunxu Wei
- 关键词：AAG ; Aspergillus niger amyloglucosidase ; ATR-FTIR ; attenuated total reflectance&ndash ; Fourier transform infrared ; 13C CP/MAS NMR ; 13C cross-polarisation magic-angle spinning nuclear magnetic resonance ; DSC ; differential scanning calorimetry ; PPA ; porcine
- 刊名：Food Chemistry
- 出版年：2014
- 出版时间：15 February, 2014
- 年：2014
- 卷：145
- 期：Complete
- 页码：245-253
- 全文大小：1735 K
文摘
Native starches were isolated from mature grains of high-amylose transgenic rice TRS and its wild-type rice TQ and treated with 0.1 % and 0.4 % NaOH for 7 and 14 days at 35 ¡ãC. Alkali-treated starches were characterised for structural and functional properties using various physical methods. The 0.1 % NaOH treatment had no significant effect on structural and functional properties of starches except that it markedly increased the hydrolysis of starch by amylolytic enzymes. The 0.4 % NaOH treatment resulted in some changes in structural and functional properties of starches. The alkali treatment affected granule morphology and decreased the electron density between crystalline and amorphous lamellae of starch. The effect of alkali on the crystalline structure including long- and short-range ordered structure was not pronounced. Compared with control starch, alkali-treated TRS starches had lower amylose content, higher onset and peak gelatinisation temperatures, and faster hydrolysis of starch by HCl and amylolytic enzymes.