Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases
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- 作者：Chenxu Zhanga ; b ; 1 ; Rongyue Zhangc ; Qiang Lia ; 1 ; qiangli@ipe.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Yongdong Huanga ; Lan Zhaoa ; Zhiguo Sua ; Fangling Gonga ; Zheng Lvd ; Hongyan Songd ; Wei Lid ; Qipeng Yuanb ; Guanghui Maa
- 关键词：Octreotide ; Reversed-phase ; Separation ; Poly(styrene&ndash ; co-divinylbenzene)
- 刊名：Separation and Purification Technology
- 出版年：2015
- 出版时间：5 November 2015
- 年：2015
- 卷：154
- 期：Complete
- 页码：351-358
- 全文大小：1204 K
文摘
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Uniformly sized PSt microspheres were synthesized by membrane emulsification technique.
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We characterized PSt microspheres and packed reversed phase column.
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Octreotide was fast separated on the PSt reversed phase column from the crude peptides.
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Spectral elucidation of purified octreotide was conducted by HPLC, FT-IR, ESI-MS and NMR.