Expression, purification and preliminary biochemical studies of the N-terminal domain of leucine-rich repeat kinase 2

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• 作者：Bin Lu ; Yujia Zhai ; Chan Wu ; Xiaoyun Pang ; Zhiheng Xu ; Fei Sun
• 关键词：Leucine-rich repeat kinase 2 ; N-terminal domain ; Ankyrin domain ; Expression and purification ; Oligomerization
• 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：1804
• 期：9
• 页码：1780-1784
• 全文大小：469 K

文摘

Leucine-rich repeat kinase 2 gene is a key factor for Parkinson's disease and encodes for a large protein kinase LRRK2 (280 kDa) with multiple domains, including the different repeat sequences at the N-terminus such as ankyrin domain. Here, we successfully expressed and purified two kinds of LRRK2's N-terminal fragments N1 (aa12–320) and N2 (aa12–860). The purified N2 protein was identified by mass spectrometry and N1's molecular weight was determined to be 33.23 kDa. Gel filtration revealed that N1 exhibits as monomer, dimer and tetramer and N2 as oligomer in solution. N1's multiple oligomeric states were further proved by native-page and cross-linking gel experiments. Circular dichroism spectrum indicated that N1 and N2 contain both α helixes and β sheets. The polymerization character of LRRK2 N-terminal region would be speculated to relate with its biological function.