Purification and immuno-gold labeling of lily mottle virus from lily leaves
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- 作者：Yubao Zhanga ; zyubao@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Yajun Wanga ; w.yajun@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Zhongkui Xiea ; wxhcas@lzb.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Ruoyu Wanga ; wangruoyu@lzb.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Zhihong Guoa ; guozhhong@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Wopke van der Werfb ; wopke.vanderwerf@wur.nl" class="auth_mail" title="E-mail the corresponding author ; Le Wanga ; lelenmg@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lily mottle virus ; Purification ; Transmission electron microscope ; Immuno-gold labeling assay
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：232
- 期：Complete
- 页码：33-38
- 全文大小：1237 K
文摘
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An efficient procedure was developed for purification of LMoV from infected leaves.
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Viral purity & integrity were confirmed by OD260:280, antibodies, PCR and TEM.
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An IGL assay was successfully applied to identify a lily virus for the first time.
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The IGL is the label viz. goat anti-rabbit 15 nm colloidal gold.