In vitro studies on human periodontal ligament stem cell sheets enhanced by enamel matrix derivative
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- 作者：Zhongshan Wang1 ; Zhihong Feng1 ; Guofeng Wu ; Shizhu Bai ; Yan Dong ; Yimin Zhao ; zhaoymdentist@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Periodontal ligament ; Stem cells ; EMD ; Proliferation ; Differentiation
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：2016
- 出版时间：1 May 2016
- 年：2016
- 卷：141
- 期：Complete
- 页码：102-111
- 全文大小：4453 K
文摘
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Enamel matrix derivative (EMD)-enhanced cell sheets were induced by complete medium supplemented with 50 μg/mL vitamin C and 100 μg/mL EMD.
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EMD-enhanced cell sheets appeared thicker and more compact than normal PDLSC sheets.
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EMD-enhanced cell sheets demonstrated more layers of cells (3–7 layers) and secreted richer extracellular matrix (ECM) than did normal periodontal ligament stem cell (PDLSC) sheets.
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EMD-enhanced cell sheets induced relatively higher mRNA expression of periodontal tissue-specific genes (COL I, POSTN), calcification-related genes (RUNX2, OPN, OCN) and a cementum tissue-specific gene (CAP) to varying degrees.
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EMD-enhanced cell sheets possessed a better mineralization ability in terms of osteogenic differentiation in vitro.