Chemiluminescence immunoassay based on dual signal amplification strategy of Au/mesoporous silica and multienzyme functionalized mesoporous silica

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• 作者：Jiehua Lin  ; ;   ; ^{linjiehua@qust.edu.cn} ; Yue Zhao ; Zhijing Wei ; Wei Wang
• 关键词：Chemiluminescent immunoassay ; Mesoporous silica ; Horseradish peroxidase ; ¦Á-Fetoprotein
• 刊名：Materials Science and Engineering: B
• 出版年：2011
• 出版时间：15 November 2011
• 年：2011
• 卷：176
• 期：18
• 页码：1474-1478
• 全文大小：778 K

文摘

A chemiluminescent dual signal amplification strategy for the determination of ¦Á-fetoprotein (AFP) was proposed based on a sandwich immunoassay format. Monoclonal antibody of AFP immobilized on the gold nanoparticles doped mesoporous SiO₂ (Au/SiO₂) were prepared and used as a primary antibody. Horseradish peroxidase (HRP) and HRP-labeled secondary antibody (Ab₂) co-immobilized into the mesoporous SiO₂ nanoparticles (HRP¨CAb₂/SiO₂) were used as the labeled immunological probe. Due to the high ratio surface areas and pore volumes of the mesoporous SiO₂, not only the amount of AFP monoclonal antibody but also the amount of the modified HRP and Ab₂ in HRP¨CAb₂/SiO₂ were largely increased. Thus the chemiluminescent signal was amplified by using the system of luminol and H₂O₂ under the catalysis of HRP. Under the optimal conditions, two linear ranges for AFP were obtained from 0.01 to 0.5 ng mL^? and 0.5 to 100 ng mL^? with a detection limit of 0.005 ng mL^? (3¦Ò). The fabricated signal amplification strategy showed an excellent promise for sensitive detection of AFP and other tumor markers.