Increased expression of Tim-3 and its ligand Galectin-9 in rat allografts during acute rejection episodes

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• 作者：Xinwei Qiao ; Ke Jiang ; Jun Nie ; Kai Fan ; Zhikun Zheng ; Jianjun Wang ; ^{bgjjww@yahoo.com.cn" class="auth_mail} ; Jinsong Li ; ^{lijinsong67@hotmail.com" class="auth_mail}
• 关键词：Lung transplantation ; Tim-3 ; Galectin-9 ; Acute rejection
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：14 March, 2014
• 年：2014
• 卷：445
• 期：3
• 页码：542-548
• 全文大小：2318 K

文摘

The aim of the study is to elucidate the profiles of T-cell immunoglobulin and mucin domain-3 (Tim-3) and its ligand Galecin-9 in acute pulmonary rejection by using a rat model of lung transplantation. Left lung grafts retrieved from Lewis or Fisher 344 rats were orthotopically transplanted into Lewis recipients without any immunosuppressions; the grafts were harvested at day 3, 7 or 10 after transplantation. The grade of acute rejection was histopathologically evaluated. Tim-3, Galectin-9, immune antigen and related cytokines expression were assessed with immunological techniques and real-time polymerase chain reaction (RT-PCR), respectively. Then, our results showed that Tim-3 and its ligand Galectin-9 were markedly up-regulated at protein and mRNA levels in allografts compared with syngrafts. Meanwhile, the decreased CD4/CD8 ratio was associated with acute rejection occurring and Tim-3 expression on CD4⁺ and CD8⁺ T cells in allografts was increased. Therefore, our study firstly described that enhanced Tim-3 and its ligand Galectin-9 in allografts might play an important role in the pathogenesis of rat lung transplant rejection, implying new valuable markers for detecting acute allograft rejection.