- 作者：Xin Lian ; Xiuzhi Duan ; Xiuling Wu ; Chengdi Li ; Shengye Chen ; Siqi Wang ; Yong Cai ; Zhiliang Weng
- 刊名：Urology
- 出版年：2012
- 出版时间：August, 2012
- 年：2012
- 卷：80
- 期：2
- 页码：485.e7-485.e13
- 全文大小：1755 K
Objective
To investigate the expression of Jade-1 and ¦Â-catenin and their effect in the development of renal cell carcinoma (RCC).Methods
The expression of Jade-1 and ¦Â-catenin in 11 normal kidney tissue specimens (normal group) and 60 RCC specimens (RCC group) was determined using real-time polymerase chain reaction and immunohistochemistry. Also, their effect on early relapses of RCC was analyzed after 5 years of follow-up.
Results
The expression of Jade-1 protein in the RCC group was significantly lower than that in the normal group (0.1655 vs 0.7438, P < .05), and the expression of ¦Â-catenin protein was significantly greater than that in the normal group (0.2756 vs 0.0855, P < .05). The expression of Jade-1 mRNA in the RCC group was 0.202 times that in the normal group, which were lower (P < .05). The expression of ¦Â-catenin mRNA was 1.014 times that in the normal group (P > .05). The expression of Jade-1 protein and ¦Â-catenin protein in poorly differentiated RCC specimens was significantly lower and higher than the expression in the well-differentiated RCC specimen (P < .05), respectively. Patients with negative Jade-1 expression and positive ¦Â-catenin expression were found to have shorter survival on univariate analysis (P < .05).
Conclusion
RCC with a low expression of Jade-1 and high expression of ¦Â-catenin is associated with a poor outcome and decreased survival.