A single marker choice strategy in simultaneous characterization and quantification of multiple components by rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS)
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- 作者：Zhangchi Ninga ; 1 ; Zhenli Liub ; 1 ; Zhiqian Songb ; Siyu Zhaoa ; Yunzhuo Dongb ; Honglian Zenga ; Yisong Shua ; Cheng Luc ; lv_cheng0816@163.com" class="auth_mail" title="E-mail the corresponding author ; Yuanyan Liua ; yyliu_1980@163.com" class="auth_mail" title="E-mail the corresponding author ; Aiping Lud ; aipinglu@hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author
- 关键词：SSDMC ; quantitative analysis of multi-components with single marker ; RRLC-QqQ-MS ; rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry ; PCA ; principal component analysis ; PLS-DA ; partial least squares discriminant analysis ; TCMs ; Traditional Chinese medicines ; RCFs ; relative correction factors ; MRM ; multiple reaction monitoring ; ERS ; exogenous reference standard ; IRS ; internal reference standard ; G-SR ; group single reference ; F-SR ; final single marker ; L
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：30 May 2016
- 年：2016
- 卷：124
- 期：Complete
- 页码：174-188
- 全文大小：3413 K
文摘
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SSDMC method is an efficient technique for quality control of TCMs.
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RRLC-QqQ-MS methods were firstly applied in SSDMC analysis.
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An improved strategy for the choice of single marker in SSDMC is proposed.
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Choice of quantitative ion is the main factor influencing the accuracy of assay.
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SSDMC coupled with statistical analysis was used to discriminate 5 Panax plants.