DNA demethylation during Chrysanthemum floral transition following short-day treatment

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• 作者：Zhongai Li¹ ; Jie Li¹ ; Yanhua Liu ; Zicheng Wang ; ^{wzc@henu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chrysanthemum ; DNA methylation ; Floral transition ; Short day
• 刊名：Electronic Journal of Biotechnology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：21
• 期：Complete
• 页码：77-81
• 全文大小：579 K

文摘

Analytical techniques such as methylation-sensitive amplification polymorphism and high-performance liquid chromatography were used to detect variation in DNA methylation of mature Chrysanthemum leaves during the floral transition induced by short-day (SD) treatment.

Results

For both early- and late-flowering cultivars, the time from the date of planting to the appearance of the capitulum bud and early blooming were significantly shorter than those of the control. The capitulum development of the early-flowering cultivar was significantly accelerated compared to the control, unlike the late-flowering cultivar. The DNA methylation percentage of leaves was significantly altered during flower development. For the early-flowering cultivar, DNA methylation was 42.2–51.3% before the capitulum bud appeared and 30.5–44.5% after. The respective DNA methylation percentages for the late-flowering cultivar were 43.5–56% and 37.2–44.9%.

Conclusions

The DNA methylation percentage of Chrysanthemum leaves decreased significantly during floral development. The decline in DNA methylation was elevated in the early-flowering cultivar compared with the late-flowering cultivar.