Determination of asperosaponin VI and its active metabolite hederagenin in rat tissues by LC-MS/MS: Application to a tissue distribution study
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- 作者：Run Zhang ; He Zhu ; Li Ding ; dinglidl@hotmail.com" class="auth_mail ; dinglihg@sina.com" class="auth_mail ; Zhonglin Yang
- 关键词：Asperosaponin VI ; Hederagenin ; LC&ndash ; MS/MS ; Tissue distribution
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：15 May 2014
- 年：2014
- 卷：959
- 期：Complete
- 页码：22-26
- 全文大小：818 K
文摘
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Simple, selective and reliable LC–MS/MS methods have been developed and validated.
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We investigated the tissue distribution of asperosaponin VI and hederagenin in rat first.
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We solved the matrix effect in tissue samples by using two gradient elution programs.
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Optimal assay conditions gave detection levels in the range of 2–30,000 ng/mL.