Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro

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• 作者：Yunxia Deng^a ; ¹ ; Dongxia Shi^a ; Zhongqiong Yin^a ; ^c ; ¹ ; ^{yinzhongq@163.com} ; Jianhong Guo^b ; Renyong Jia^a ; ^c ; Jiao Xu^a ; ¹ ; Xu Song^a ; Cheng Lv^a ; Qiaojia Fan^a ; Xiaoxia Liang^a ; Fei Shi^a ; Gang Ye^a ; Wei Zhang^a
• 关键词：Sarcoptes scabiei ; Acaricidal activity ; Neem oil ; In vitro
• 刊名：Experimental Parasitology
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：130
• 期：4
• 页码：475-477
• 全文大小：223 K

文摘

The petroleum ether extract of neem oil and its four fractions separated by column chromatography was diluted at different concentrations with liquid paraffin. The acaricidal bioassay was conducted using a dipping method. The results indicated that the median lethal concentration (LC50) of the petroleum ether extract (at the concentration of 500.0 ml/l) was 70.9 ml/l, 24 h after treatment. At concentrations of 500.0, 250.0, 125.0, 62.5 and 31.2 ml/l, the median lethal times (LT50) of the petroleum ether extract were 8.7, 8.8, 10.8, 11.5 and 13.1 h, respectively. Thin-layer chromatography (TLC) showed that the petroleum ether extract of neem oil separated into four fractions (F1-F4). Acaricidal activity of 68.3 % and 100.0 % in the F2 and F4 was confirmed. These results suggest that petroleum ether extracts of neem oil and its four fractions possess useful acaricidal activity in vitro.