A specific antimicrobial protein CAP-1 from Pseudomonas sp. isolated from the jellyfish Cyanea capillata

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• 作者：Manman Yin^a ; ^b ; ¹ ; ^{mmy19900215@163.com" class="auth_mail" title="E-mail the corresponding author} ; Dan Liu^a ; ^b ; ¹ ; ^{liudanzhongyao@163.com" class="auth_mail" title="E-mail the corresponding author} ; Feng Xu^c ; ¹ ; ^{xufeng19880424@126.com" class="auth_mail" title="E-mail the corresponding author} ; Liang Xiao^a ; ^b ; ^{hormat830713@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Qianqian Wang^a ; ^b ; ^{abc_w@163.com" class="auth_mail" title="E-mail the corresponding author} ; Beilei Wang^a ; ^b ; ^{lilly_wang@126.com" class="auth_mail" title="E-mail the corresponding author} ; Yinlong Chang^a ; ^{donlone@yeah.net" class="auth_mail" title="E-mail the corresponding author} ; Jiemin Zheng^a ; ^b ; ^{jmzheng@smmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Xia Tao^d ; Guoyan Liu^a ; ^b ; ^{lgy_laurie@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Liming Zhang^a ; ^b ; ^{lmzhang@smmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antimicrobial protein ; Antimicrobial activity ; Cyanea capillata
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：82
• 期：Complete
• 页码：488-496
• 全文大小：1737 K

文摘

A bacterium strain, designated as CMF-2, was isolated from the jellyfish Cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. The strain CMF-2 was identified as Pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16S rRNA sequence analysis. In this study, an antimicrobial protein, named as CAP-1, was isolated from the culture of CMF-2 through ammonium sulfate precipitation and gel filtration chromatography. According to the result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), a major band indicated that the antimicrobial protein had a molecular mass of about 15 kDa, and it was identified as a hypothetical protein by MALDI-TOF-MS analysis and Mascot searching. CAP-1 displayed a broad antimicrobial spectrum against the indicator bacteria and fungus, including Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans, especially some marine-derived microorganisms such as Vibrio vulnificus, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio cholera, and Vibrio anguillarum, but showed little impact on tumor cells and normal human cells. The protein CAP-1 remained a stable antimicrobial activity in a wide range of temperature (20–80 °C) and pH (2–10) conditions. These results suggested that CAP-1 might have a specific antimicrobial function not due to cytotoxicity.