Expression and purification of the kinase domain of PINK1 in Pichia pastoris
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- 作者：Daichao Wua ; Lingzhi Qua ; Yang Fub ; Jun Lia ; Longying Jianga ; Xiaojuan Chena ; Ming Guoa ; Zhuchu Chena ; Lin Chena ; b ; linchen@usc.edu" class="auth_mail" title="E-mail the corresponding author ; Yongheng Chena ; b ; yonghenc@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Heterologous expression ; PINK1 ; Pichia pastoris ; Parkinson's disease ; Ubiquitin
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：67-72
- 全文大小：894 K
文摘
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The kinase domain of PINK1 was successfully expressed in Pichia pastoris.
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The induction conditions of recombinant PINK1 transformant were optimized.
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Recombinant PINK1 was obtained after two steps purification.
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The purity of purified recombinant PINK1 was higher than 95%.
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Recombinant PINK1 was able to phosphorylate ubiquitin.