Expression of heat shock protein 90 genes during early development and infection in Megalobrama amblycephala and evidence for adaptive evolution in teleost

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• 作者：Zhujin Ding ; Junjie Wu ; Lina Su ; Fengjuan Zhou ; Xiaoheng Zhao ; Wei Deng ; Jie Zhang ; Shikai Liu ; Weimin Wang ; Hong Liu
• 关键词：Megalobrama amblycephala ; Heat shock protein 90 ; Aeromonas hydrophila ; Teleost ; Adaptive selection
• 刊名：Developmental and Comparative Immunology
• 出版年：2013
• 出版时间：December, 2013
• 年：2013
• 卷：41
• 期：4
• 页码：683-693
• 全文大小：4944 K

文摘

Heat shock protein 90 (HSP90) are highly conserved molecular chaperones, playing a pivotal role in cellular progress. In this study, we reported the characterization of the Hsp90¦Á and Hsp90¦Â genes in Megalobrama amblycephala, the expression profiling during early development, in various healthy tissues and in response to bacterial infection, and the assessment of their adaptive evolution. The Hsp90¦Á cDNA contains an open reading frame (ORF) of 2193 bp encoding 731 amino acids and the Hsp90¦Â cDNA has an ORF of 2184 bp encoding 728 amino acids. Using quantitative real-time PCR (qRT-PCR) analysis, the mRNA of both Hsp90¦Á and Hsp90¦Â reached the highest level at 15th day post-hatch. Using qRT-PCR and Western blot, both Hsp90¦Á and Hsp90¦Â were widely expressed in various healthy tissues and significantly higher in blood than in other tissues. Expression of both Hsp90¦Á and Hsp90¦Â were up-regulated upon bacterial infection and reached the peak level at 4 h post infection. Site model analysis indicated that one positive selection site (T717) in Hsp90¦Á was found, while no positive selection site was observed in Hsp90¦Â. Branch-site model test showed that there were adaptively evolutionary evidences in the branches of Salmoniformes and Gasterosteiformes for Hsp90¦Á gene, and in the branch of Salmoniformes for Hsp90¦Â gene.