Sensitive and selective chemiluminescence assay for hydrogen peroxide in exhaled breath condensate using nanoparticle-based catalysis

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• 作者：Xiaohua Li ; Zhujun Zhang ; ^{zhangzj@snnu.edu.cn} ; Liang Tao ; Miao Gao
• 关键词：Co3O4 nanoparticles ; ¦Á-Fe2O3 nanorods ; NiO nanoparticles ; Hydrogen peroxide ; Exhaled breath condensate
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：107
• 期：Complete
• 页码：311-316
• 全文大小：900 K

文摘

The catalytic properties of cubiform Co₃O₄ nanoparticles, ¦Á-Fe₂O₃ nanorods, and NiO nanoparticles were studied using both microarray method and FI-CL method. These nanoarticles exhibit high specific catalytic effects on the chemiluminescence (CL) reaction of the luminol-H₂O₂ system in alkaline solution compared with other common catalysts. A reaction mechanism is described. It provides new insights into the application of nanoparticle materials. The CL method based on the use of the Co₃O₄ nanoparticles is ultrasensitive and particularly selective. Therefore, it was applied to the analysis of H₂O₂ which can be determined in the concentration range from 1.0 nM to 1000 nM, with a detection limit of 0.3 nM. The relative standard deviation is 2.1 % at 0.1 ¦ÌM of H₂O₂ (for n = 11). The method was successfully applied to the determination of trace quantities of H₂O₂ in exhaled breath condensate (EBC) where it is a mediator of oxidative stress and a promising biomarker for diagnosing. The assay requires a small sample and no incubation time, and has an analytical runtime of <1 min. It is timesaving and suitable for larger studies. The levels of H₂O₂ in EBC are found to be elevated in healthy subjects (average = 0.54 nM), rheum subjects (average = 0.24 nM), and feverish subjects (average = 0.16 nM). Our data suggested that the average H₂O₂ concentration of EBC from feverish subjects was significantly higher than healthy subjects and rheumatic subjects.