AND logic gate application based on colorimetric screening of enzyme activity

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• 作者：Ziheng Liu ; Yihong Zhan ; Yu Bai ; Jianwen Sun
• 关键词：Computational devices ; Logic gate ; DNA/Gold nanoparticle ; DNase-I
• 刊名：Solid State Sciences
• 出版年：2012
• 出版时间：July, 2012
• 年：2012
• 卷：14
• 期：7
• 页码：870-873
• 全文大小：427 K

文摘

A simple and novel DNA and gold nanoparticle (AuNPs) based colorimetric method has been developed for efficient detection of Deoxyribonuclease I (DNase-I) activity. For the detection process, the sample that contains DNase-I, which will cleave double stranded DNA into small pieces. Furthermore, the small pieces DNA sequences and water-soluble conjugated polymer cause the AuNPs aggregation (the solution change the color from red to blue). Thus, DNase-I could be detected by naked eyes or UV/Vis. Molecular substrates can be viewed as computational devices that process physical or chemical ¡®inputs¡¯ to generate ¡®outputs¡¯ based on a set of logical operators. Based on the assay, we have presented a DNA/AuNP based, multi-readout AND logic operations.