Inactivation and mechanisms of chlorine dioxide on Nosema bombycis

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• 作者：Zhengyong Wang ; Fupin Liao ; Jianrong Lin ; Wenchu Li ; Yangsheng Zhong ; Peichan Tan ; Ziran Huang
• 关键词：Nosema bombycis ; AO– ; PI stain ; Disinfectant ; Chlorine dioxide ; Mechanism
• 刊名：Journal of Invertebrate Pathology
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：104
• 期：2
• 页码：134-139
• 全文大小：1604 K

文摘

Biological tests demonstrated that the inactivation of Nosema bombycis (N. bombycis) spores by chlorine dioxide (ClO₂) occurs very fast and is highly sensitive. The lowest effective inactivation dosage and time was 15 mg/mL for 30 min. The inactivation of spores was additionally verified by using double color fluorescence stain and spore germination testing. A series of biological changes, including a large number of substrates that were leaked out from the spores included proteins, DNA, polysaccharide, K⁺, and Ca²⁺, occurred a short time after N. bombycis spores were treated with ClO₂. In addition, the lipid of spores was disrupted and ATPase activity was inhibited, which resulted in the destruction of the inner structure of the spores.