Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus

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• 作者：Huahua Du ; Zirong Xu ; Xiaofeng Wu ; Weifen Li and Wei Dai
• 关键词：White spot syndrome virus ; VP28 ; Protection ; Procambarus clarkii ; Crayfish
• 刊名：Aquaculture
• 出版年：2006
• 出版时间：29 September 2006
• 年：2006
• 卷：260
• 期：1-4
• 页码：39-43
• 全文大小：244 K

文摘

In order to investigate whether protein structure has an effect on protective effect of envelope protein of WSSV, VP28 protein was expressed both in Escherichia coli (pET-VP28) and insect (BmN) cells (BmNPV-VP28). The baculovirus (BmNPV) expression system was used to obtain correctly folded VP28 protein. Procambarus clarkii crayfish were intramuscularly injected with lysates of cells infected with recombinant pET-VP28 and BmNPV-VP28, respectively, and then challenged by intramuscular injection of WSSV to assess the duration of protection. The crayfish injected with BmNPV-VP28 showed generally lower mortality rates when compared to crayfish injected with pET-VP28, resulting in relative percent survivals of 92 % and 39 % , respectively, when compared to the control groups injected with empty vectors BmNPV and pET-30a. These results showed that VP28 protein produced in BmN cells gave much better protection than VP28 protein produced in E. coli.