Microbial source tracking using host specific FAME profiles of fecal coliforms

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• 作者：Metin Duran ; Berat Z. Haznedaroğ ; lu and Daniel H. Zitomer
• 关键词：Fecal pollution ; FAME profiling ; Microbial source tracking ; Fecal coliform ; Source-water protection
• 刊名：Water Research
• 出版年：2006
• 出版时间：January 2006
• 年：2006
• 卷：40
• 期：1
• 页码：67-74
• 全文大小：240 K

文摘

The objective of this study was to investigate the host-specific differences in fatty acid methyl ester (FAME) profiles of fecal coliforms (FC). A known-source library was constructed with 314 FC isolates cultured from 6 possible sources of fecal pollution; 99 isolates from sewage; 29 from bovine; 29 from poultry; 50 from swine; 46 from waterfowl; and 61 from deer. It was found that the hydroxy FAMEs 12:0 2OH, 12:0 3OH, and 14:0 2OH were exclusively associated with isolates of human origin. On the other hand, 3 saturated FAMEs, 10:0, 15:0, and 18:0 were found only in isolates from non-human sources, 15:0 being associated with livestock samples only. In addition to the presence of these signature FAMEs, the mean relative masses of 16:1 ω7c and 16:1 ISO/14:0 3OH were significantly different between the isolates of human and non-human origins. A linear discriminant function differentiated FC isolates of human origin from those of livestock and wildlife origin at 99 % accuracy. These results strongly suggest that the FAME profiles of FC show statistically significant host specificity and may have the potential to be used as a phenotypic microbial source tracking tool.