LncRNA expression signatures of twist-induced epithelial-to-mesenchymal transition in MCF10A cells

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• 作者：Ping Hu ; Jiajia Yang ; Yixuan Hou ; Hailong Zhang ; Zongyue Zeng ; Liuyang Zhao ; Tenghua Yu ; Xi Tang ; Gang Tu ; Xiaojiang Cui ; Manran Liu
• 关键词：Twist ; LncRNAs ; EMT ; Signal network
• 刊名：Cellular Signalling
• 出版年：January, 2014
• 年：2014
• 卷：26
• 期：1
• 页码：83-93
• 全文大小：1739 K

文摘

The epithelial-to-mesenchymal transient (EMT) is associated with tumor metastasis. Twist is one of the key transcription factors for EMT and relates to tumor cell migration. Long non-coding RNAs (lncRNAs) have recently emerged as important regulatory molecules involved in a broad range of biological processes and complicated diseases. However, it is unknown whether a signal network and lncRNAs are involved in Twist-induced EMT program. Taking MCF10A/Twist as a model, more than 99 lncRNAs and 3164 genes are regulated in the Twist-induced EMT process using lncRNA-array and cDNA micro-array. We establish a downstream signal network associated with EMT induced by Twist using bioinformatic analysis (Gene Ontology, pathway analysis) and experimental data. A set of multiple canonical signal pathways (such as WNT, MAPK, JAK/STAT, TGF-尾, mTOR, Hedgehog and P53 signaling pathways) and several lncRNAs [such as lncRNA (chr6, 26124411-26139312, +), lncRNA (chr1, 41944445-41949874, 鈭?, lncRNA (chr17, 44833874-44834830, +)] are altered in MCF10A/Twist cells. More interestingly, lncRNA (chr17, 44833874-44834830, +), lncRNA (chr17, 21142183-21156578, 鈭?, lncRNA (chr6, 26124411-26139312, +) and lncRNA (chr19, 438420-2083745, 鈭? may be involved in regulation or activation of WNT signaling pathway in the Twist-induced EMT process. These findings first determine that Twist contributes to invasion and metastasis by inducing wide-ranging transcriptional and functional changes of lncRNAs and signal pathways in our study.