Glycation of β-lactoglobulin under dynamic high pressure microfluidization treatment: Effects on IgE-binding capacity and conformation
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- 作者：Yuan Chena ; Zongcai Tua ; b ; tuzc_mail@aliyun.com" class="auth_mail" title="E-mail the corresponding author ; Hui Wangb ; wanghui00072@aliyun.com" class="auth_mail" title="E-mail the corresponding author ; Lu Zhanga ; Xiaomei Shaa ; Juanjuan Panga ; Ping Yanga ; Guangxian Liua ; Wenhua Yangb
- 关键词：β-Lg ; β-lactoglobulin ; DHPM ; dynamic high-pressure microfluidization ; IgE ; immunoglobulin E ; HPP ; high hydrostatic pressure processing ; CMA ; cow's milk allergy ; CD ; circular dichroism ; SDS ; sodium dodecyl sulphate ; ANS ; 1-anilinonaphthalene-8-sulfonate ; ELISA ; enzyme-linked immunosorbent assay ; PBS ; phosphate buffer solution ; PBST ; PBS/Tween solution ; CBS ; carbonate buffer solution ; H0 ; surface hydrophobicity ; SDS-PAGE ; Sodium dodecyl sulphate polyacrylamide gel electrophoresis
- 刊名：Food Research International
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：89
- 期：part_P1
- 页码：882-888
- 全文大小：436 K
文摘
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The IgE-binding capacity of β-Lg after glycation and DHPM treatment was studied.
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Glycation combined with DHPM caused the unfolding and aggregation of β-Lg, follow by increased denaturation temperature.
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DHPM treatment decreased the IgE-binding capacity of β-Lg-galactose conjugates.