Development of propidium iodide as a fluorescence probe for the on-line screening of non-specific DNA-intercalators in Fufang Banbianlian Injection
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- 作者：Yanyan Niua ; 1 ; Sensen Lia ; 1 ; Zongtao Linb ; Meixian Liua ; Daidong Wanga ; Hong Wanga ; ; Shizhong Chena ; chenshizhong66@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DNA binding activity ; Fufang banbianlian injection ; Fluorescence detector ; HPLC&minus ; DAD&minus ; MS ; On-line method ; Propidium iodide
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：9 September 2016
- 年：2016
- 卷：1463
- 期：Complete
- 页码：102-109
- 全文大小：1047 K
文摘
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Propidium iodide probe was developed into an on-line screening method for the first time.
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HPLC−DAD−MSn−DNA−PI−FLD system was established for screening active compounds.
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PI was able to identify non-specific DNA intercalators from complex samples.
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38 of the identified compounds are DNA intercalation active in Fufang Banbianlian Injection.