Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits
详细信息 查看全文
- 作者：Huimin Guoa ; Jie Zhua ; Yonggui Tana ; Chuanfeng Lia ; Zongyan Chena ; Shiqi Sunb ; sunshiqi@caas.cn" class="auth_mail" title="E-mail the corresponding author ; Guangqing Liua ; liugq@shvri.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Rabbit hemorrhagic disease virus ; VP60 gene ; Virus-like particle ; Vaccine
- 刊名：Antiviral Research
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：131
- 期：Complete
- 页码：85-91
- 全文大小：1108 K
文摘
- listitem" id="list_ulist0010">
- •
Soluble RHDV-VP60 protein was expressed in Escherichia coli by a pSMK vector containing a SUMO tag.
- •
The VP60 protein of RHDV has self-assembled into VLPs after the cleavage of the SUMO tag.
- •
RHDV specific responses were obviously induced in rabbits by RHDV-VLPs.
- •
VLPs-based RHDV vaccine can be used as a promising RHDV vaccine candidate.