Transglucosylation of hydroquinone catalysed by α-glucosidase from baker's yeast

详细信息    查看全文

• 作者：Radivoje Prodanović ; Nenad Milosavić ; Duš ; an Sladić ; Mario Zlatović ; Branislav Bož ; ić ; Tanja Ć ; irković ; Veli&#x10d ; ković ; and Zoran Vuj&#x10d ; i&#
• 关键词：α ; -Arbutine ; Hydroquinone ; Maltase ; Isomaltoside ; Transglycosylation
• 刊名：Journal of Molecular Catalysis B: Enzymatic
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：35
• 期：4-6
• 页码：142-146
• 全文大小：188 K

文摘

Hydroquinone α-isomaltoside and hydroquinone α-glucoside were synthesized by transglucosylation in an aqueous system with baker's yeast α-glucosidase from hydroquinone and maltose as a glucosyl donor. Only one phenolic group was glucosylated, with α-selectivity, and the nature of the reaction products was governed by the concentration of hydroquinone. The optimal conditions for synthesis of glycosides were 9 mM hydroquinone and 1.5 M maltose in a 100 mM sodium citrate/phosphate buffer at pH 5.0 and 30 °C for 20 h. Under these conditions both hydroquinone α-glycosides were obtained in nearly equimolar amounts with a total molar yield of 28 % with respect to hydroquinone and a total glycoside concentration of 1 mg/mL in the reaction mixture.