- 作者：Rosa Virginia Garc铆a-Rodr铆guez ; Gabriel Alfonso Guti茅rrez-Rebolledo ; Enrique M茅ndez-Bolaina ; Alberto S谩nchez-Medina ; Octavio Maldonado-Saavedra ; Miguel 脕ngel Dom铆nguez-Ortiz ; Maribel V谩zquez-Hern谩ndez ; Omar David Mu帽oz-Mu帽iz ; Jes煤s Samuel Cruz-S谩nchez
- 关键词：Antioxidant ; Anti-inflammatory ; Cardioprotective ; Acute toxicity ; Cnidoscolus chayamansa ; Mexican traditional medicine
- 刊名：Journal of Ethnopharmacology
- 出版年：3 February, 2014
- 年：2014
- 卷：151
- 期：2
- 页码：937-943
- 全文大小：351 K
Ethnopharmacological relevance
Cnidoscolus chayamansa Mc Vaugh (Euphorbiaceae) is commonly known as 鈥榗haya鈥?in Central America. In South East Mexico, because of its high nutritional values, is an important part of the diet of many indigenous communities. Chaya is also used as a traditional remedy for the treatment of diabetes, rheumatism, gastrointestinal disorders and inflammation-related diseases. Although Cnidoscolus chayamansa is one of most used and valued medicinal plants, only few studies on documenting its pharmacological properties can be found.Materials and methods
Dried leaves of Cnidoscolus chayamansa were subjected to a successive maceration using Hex, EtOAc and EtOH. The antioxidant activities of the extracts were tested using the DPPH radical scavenging, Ferric reducing/antioxidant power and total phenolic content assays. To determine the anti-inflammatory activity, the TPA-induced mouse ear edema and the carrageenan-induced mouse paw edema assays were used. The cardioprotective effects of the EtOH extract was determined using the ischemia/reperfusion (I/R) rat model. Finally, the acute toxicity was determined using Lorke's method.
Results
The results showed a similar anti-inflammatory activity (鈮?0%) for all extracts but only the EtOAc extract showed relevant activity when applied intraperitoneally. When tested for their antioxidant activity none of the extracts showed a significant activity suggesting that the antinflammatory activity is not related to a direct free radical scavenging of the extracts. Additionally, the EtOH extract showed a strong cardioprotective effect at 500 mg/kg when given orally. Both the EtOAc and the EtOH extract have a LD50 >5 g/kg, confirming their safety in acute oral administration.
Conclusions
All these results are relevant for a better understanding of the therapeutic used of Cnidoscolus chayamansa in the Mexican traditional medicine and highlights its cardioprotective potential.