Antibody-coupled hydroxyapatite nanoparticles as efficient tools for labeling intracellular proteins

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• 作者：Fabiola Vá ; zquez-Herná ; ndez^a ; ¹ ; ^{fvazquez_84@yahoo.com.mx} ; ^{fvazquez@cinvestav.mx} ; Salvador Mendoza-Acevedo^b ; ² ; ^{smendozaa10@gmail.com} ; Claudia Oliva Mendoza-Barrera^c ; ^{cmendoza_barrera@hotmail.com} ; Julio Mendoza-Á ; lvarez^d ; ^{juliogma1@gmail.com} ; Juan Pedro Luna-Arias^a ; ^e ; ^{jpluna2003@gmail.com} ; ^{jpluna@cell.cinvestav.mx} ; ^{jpluna@cinvestav.mx}
• 关键词：Bioconjugation ; Cell viability ; Functionalization ; Hydroxyapatite ; Immunofluorescence ; Nanoparticle
• 刊名：Materials Science and Engineering: C
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：71
• 期：Complete
• 页码：909-918
• 全文大小：2344 K
• 卷排序：71

文摘

A modified hydrothermal method to synthesize hydroxyapatite nanoparticles is used. A protocol to functionalize and bioconjugate these nanoparticles is proposed. Once functionalized, they affected breast cancer cell lines viability. Once bioconjugated, they labeled intracellular proteins. Protocol is more efficient than traditional immunofluorescence labeling method.