Cell migration or cytokinesis and proliferation? - Revisiting the 鈥済o or grow鈥?hypothesis in cancer cells in vitro

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• 作者：Tam谩s Garay ; 脡va Juh谩sz ; Eszter Moln谩r ; Maria Eisenbauer ; Andr谩s Czir贸k ; Barbara Dekan ; Vikt贸ria L谩szl贸 ; Mir Alireza Hoda ; Bal谩zs D枚me ; J贸zsef T铆m谩r ; Walter Klepetko ; Walter Berger ; Bal谩zs Heged疟s
• 关键词：Migration ; Proliferation ; Cytokinesis ; Melanoma ; Mesothelioma ; Lung cancer
• 刊名：Experimental Cell Research
• 出版年：10 December, 2013
• 年：2013
• 卷：319
• 期：20
• 页码：3094-3103
• 全文大小：2895 K

文摘

The mortality of patients with solid tumors is mostly due to metastasis that relies on the interplay between migration and proliferation. The 鈥済o or grow鈥?hypothesis postulates that migration and proliferation spatiotemporally excludes each other.

We evaluated this hypothesis on 35 cell lines (12 mesothelioma, 13 melanoma and 10 lung cancer) on both the individual cell and population levels. Following three-day-long videomicroscopy, migration, proliferation and cytokinesis-length were quantified. We found a significantly higher migration in mesothelioma cells compared to melanoma and lung cancer while tumor types did not differ in mean proliferation or duration of cytokinesis. Strikingly, we found in melanoma and lung cancer a significant positive correlation between mean proliferation and migration. Furthermore, non-dividing melanoma and lung cancer cells displayed slower migration. In contrast, in mesothelioma there were no such correlations. Interestingly, negative correlation was found between cytokinesis-length and migration in melanoma. FAK activation was higher in melanoma cells with high motility.

We demonstrate that the cancer cells studied do not defer proliferation for migration. Of note, tumor cells from various organ systems may differently regulate migration and proliferation. Furthermore, our data is in line with the observation of pathologists that highly proliferative tumors are often highly invasive.