Antibody functionalization with a dual reactive hydrazide/click crosslinker

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• 作者：Hoa Thi Le¹ ; Jae-Gyu Jang¹ ; Ji Young Park ; Choon Woo Lim ; ^{choonwoo.lim@khu.ac.kr} ; Tae Woo Kim ; ^{tw1275@khu.ac.kr}
• 关键词：Antibody functionalization ; Hydrazide chemistry ; Cu(I)-catalyzed azide&ndash ; alkyne cycloaddition ; Dual reactive hydrazide/click crosslinker ; Labeling
• 刊名：Analytical Biochemistry
• 出版年：2013
• 出版时间：1 April, 2013
• 年：2013
• 卷：435
• 期：1
• 页码：68-73
• 全文大小：763 K

文摘

A water-soluble, dual reactive hydrazide/click crosslinker (ethynyl hydrazide, EH) was synthesized and characterized. A model antibody, human immunoglobulin G (hIgG), was ethynylated by conventional oxidation/hydrazide reactions with the hydrazide moiety of EH. The terminal alkyne conjugated to the glycan of hIgG was easily functionalized by quantitative and bioorthogonal Cu(I)-catalyzed azide-alkyne cycloaddition. The potential of the hydrazide/click crosslinker as a reagent to functionalize antibodies was demonstrated with fluorophore labeling and antibody immobilization.