Circulating microRNAs and sudden cardiac arrest outcomes

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• 作者：P.L. Wander^a ; ^e ; ^{lwander@u.washington.edu" class="auth_mail" title="E-mail the corresponding author} ; D.A. Enquobahrie^b ; C.C. Pritchard^c ; B. McKnight^d ; K. Rice^d ; M. Christiansen^a ; R.N. Lemaitre^a ; T. Rea^a ; ^f ; D. Siscovick^g ; N. Sotoodehnia^a ; ^b ; ^h
• 关键词：Cardiac arrest ; Outcomes ; Epigenetics ; microRNAs ; Epidemiology
• 刊名：Resuscitation
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：106
• 期：Complete
• 页码：96-101
• 全文大小：434 K

文摘

MicroRNAs (miRNAs) have regulatory functions in organs critical in resuscitation from sudden cardiac arrest due to ventricular fibrillation (VF-SCA); therefore, circulating miRNAs may be markers of VF-SCA outcome.

Methods

We measured candidate miRNAs (N = 45) in plasma using qRT-PCR among participants of a population-based VF-SCA study. Participants were randomly selected cases who died in the field (DF, n = 15), died in hospital (DH, n = 15), or survived to discharge (DC, n = 15), and, age-, sex-, and race-matched controls (n = 15). MiRNA levels were compared using ANOVA, t-tests, and fold-changes.

Results

Mean age of groups ranged from 66.9 to 69.7. Most participants were male (53–67%) and white (67%). Comparing cases to controls, plasma levels of 17 miRNAs expressed in heart, brain, liver, and other tissues (including miR-29c, -34a, -122, -145, -200a, -210, -499-5p, and -663b) were higher and three non-specific miRNAs lower (miR-221, -330-3p, and -9-5p). Among DH or DC compared with DF cases, levels of two miRNAs (liver-specific miR-122 and non-specific miR-205) were higher and two heart-specific miRNAs (miR-208b and -499-5p) lower. Among DC vs. DF cases, levels of three miRNAs (miR-122, and non-specific miR-200a and -205) were higher and four heart-specific miRNAs (miR-133a, -133b, -208b, and -499-5p) lower. Among DC vs. DH cases, levels of two non-specific miRNAs (miR-135a and -9-3p) were lower.

Conclusions

Circulating miRNAs expressed in heart, brain, and other tissues differ between VF-SCA cases and controls and are related to resuscitation outcomes. Measurement of miRNAs may clarify mechanisms underlying resuscitation, improve prognostication, and guide development of therapies. Results require replication.