Analytical measurement and clinical relevance of vitamin D₃ C3-epimer

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• 作者：Dana Bailey^a ; Kika Veljkovic^b ; Mehrdad Yazdanpanah^c ; Khosrow Adeli^a ; ^c ; ^{khosrow.adeli@sickkids.ca}
• 关键词：1¦Á ; 25(OH)D3 ; 1¦Á ; 25-dihydroxy-vitamin D3 ; 25(OH)D3 ; 25-hydroxy-vitamin D3 ; 25(OH)D2 ; 25-hydroxy-vitamin D2 ; 3-epi-25(OH)D3 ; 25-hydroxy-3-epi-vitamin D3 ; 3-epi-1¦Á ; 25(OH)D3 ; 1¦Á ; 25-dihydroxy-3-epi-vitamin D3 ; DEQAS ; Vitamin D External Quality Assessment S
• 刊名：Clinical Biochemistry
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：46
• 期：3
• 页码：190-196
• 全文大小：463 K

文摘

With an ever-increasing clinical interest in vitamin D insufficiency, numerous automated immunoassays, protein binding assays, and in-house LC-MS/MS methods are being developed for the quantification of 25-hydroxyvitamin D₃ (25(OH)D₃). Recently, LC-MS/MS methods have identified an epimeric form of 25(OH)D₃ that has been shown to contribute significantly to 25(OH)D₃ concentration, particularly in infant populations. This review describes the metabolic pathway and physiological functions of 3-epi-vitamin D, compares the capability of various 25(OH)D₃ methods to detect the epimer, and highlights recent publications quantifying 3-epi-25(OH)D₃ in infant, pediatric, and adult populations. In total, this review summarizes the information necessary for clinicians and laboratorians to decide whether or not to report/consider the C3-epimer in the analysis and clinical assessment of vitamin D status.