Recombinant expression, characterization, and application of a phospholipase B from Fusarium oxysporum

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• 作者：Lingqia Su^a ; ^b ; Dening Ji^a ; ^b ; Xiumei Tao^a ; ^b ; Lingang Yu^a ; ^b ; Jing Wu^a ; ^b ; ^{jingwu@jiangnan.edu.cn} ; Yongmei Xia^a
• 关键词：Phospholipase B ; Pichia pastoris ; Overexpression ; Enzyme characterization ; Oil degumming
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：92-100
• 全文大小：1378 K
• 卷排序：242

文摘

The Fusarium oxysporum putative lipase was identified as a PLB. The PLB activity obtained (6503.8 U mL−1) is the highest reported to date. The PLB reduced the phosphorus content of a crude oil from 75.88 ppm to 3.3 ppm. This study provided insights into production and application of the F. oxysporum PLB.