Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis

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• 作者：Xiumei Wei^a ; Jianmin Yang^a ; Dinglong Yang^a ; Jie Xu^a ; Xiangquan Liu^a ; ^{lxq6808@163.com} ; Jialong Yang^b ; ^{jlyang@yic.ac.cn} ; Jinghui Fang^a ; Hongjin Qiao^a
• 关键词：Solen grandis ; PGRP ; Innate immunity ; Pattern recognition receptor ; Real-time PCR
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：32
• 期：1
• 页码：178-185
• 全文大小：1580 K

文摘

Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672?and 1285?bp, containing an open reading frame (ORF) of 813 and 426?bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn²⁺ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by ¦Â-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly up-regulated (P?<?0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders.