Bacterial viability was reduced after exposure to curcumin at 75 μM and LED light doses of 139 J/cm2, 278 J/cm2 and 417 J/cm2. Aeromonas hydrophila displayed more sensibility to the treatment than other Gram negative bacteria. Pseudomonas aeruginosa was the most resistant to photodynamic inactivation mediated by curcumin.