Studying nuclear functions of aminoacyl tRNA synthetases

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• 作者：Yi Shi^a ; ^b ; ^{yishi@scripps.edu} ; ^{yishi@nankai.edu.cn} ; Na Wei^a ; Xiang-Lei Yang^a ; ^{xlyang@scripps.edu}
• 关键词：Aminoacyl tRNA synthetase ; Nuclear function ; Transcriptional regulation cell fractionation ; Chromatin immunoprecipitation
• 刊名：Methods
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：113
• 期：Complete
• 页码：105-110
• 全文大小：775 K
• 卷排序：113

文摘

Methods for studying nuclear function of AARS are proposed. Cell fractionation used for determining the nuclear localization of AARS is described. DNA-cellulose pull-down assay for analyzing the direct interaction of AARS with DNA is provided. Chromatin immunoprecipitation assay for identifying the AARS binding loci in the genome is described in detail.